Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Product Name | PRMT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRMT5, JBP1, SKB1, IBP72, SKB1Hs, HRMT1L5 |
| Product description | PRMT5 methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). It methylates SUPT5H. PRMT5 plays a role in the assembly of snRNP core particles and may play a role in cytokine-activated transduction pathways. It negatively regulates cyclin E1 promoter activity and cellular proliferation and May regulate the SUPT5H transcriptional elongation properties. It may be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. PRMT5 methylates histone H2A/H4 'Arg-3' during germ cell development and methylates histone H3 'Arg-8', which may repress transcription.1) Azzouz, T.N., (2005) J. Biol. Chem. 280 (41), 34435-34440. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRMT5 |
|---|---|
| Alternative Names | PRMT5 JBP1 SKB1 IBP72 SKB1Hs HRMT1L5 |
| Molecular Weight(MW) | 68 kDa, 73 kDa |
| Entrez Gene | 10419 |
|---|---|
| Protein Accession | NP_001034708 |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Application
Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PRMT5 antibody can be used for detection of PRMT5 by ELISA at 1:62500. PRMT5 antibody can be used for detection of PRMT5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PRMT5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
