Application
PRMT5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PRMT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally |
| Product description | Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.1) Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010) |
| Immunogen | This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRMT5 |
|---|---|
| Alternative Names | Protein arginine N-methyltransferase 5 211- 72 kDa ICln-binding protein Histone-arginine N-methyltransferase PRMT5 Jak-binding protein 1 Shk1 kinase-binding protein 1 homolog SKB1 homolog SKB1Hs Protein arginine N-methyltransferase 5 N-terminally |
| Molecular Weight(MW) | 73 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
PRMT5 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
