Application
Western blot analysis of lysed extracts from 293 cells using Raf1 (Ab-338).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Ab-338).| Product Name | Raf1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.1) Adams DG, et al. (2005) J Biol Chem. 280 (52): 42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306 (4): 924-9. Noser JA, et al. (2007) Mol Ther. 15 (8): 1531-6. |
| Immunogen | Raf1 (Ab-338) antibody was raised against a peptide sequence around aa.336~340 (R-D-S-S-Y) derived from Human RAF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Alternative Names | NS5 CRAF Raf-1 c-Raf ESR NR3A1 C-RAF C-Raf |
| Molecular Weight(MW) | 73 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Raf1 protein. |
| Entrez Gene | 5894 |
|---|---|
| Protein Accession | NP_002871.1 |
Application
Western blot analysis of lysed extracts from 293 cells using Raf1 (Ab-338).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Ab-338).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
