Application
Western blot analysis of lysed extracts from 293 and MDA cells untreated or treated with PMA using Raf1 (Phospho-Ser259).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Phospho-Ser259).| Product Name | Raf1 (phospho Ser259) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.1) Dougherty M K, et al. (2005) Mol Cell. 17 (2): 215-224. Hekman M, et al. (2005) FEBS Lett. 579 (2): 464-468. Avruch J, et al. (1994) Trends Biochem. Sci. 19, 279-283. Chong H, et al. (2001) EMBO J. 20, 3716-3727. King A J, et al. (1998) Nature. 396:180-183. |
| Immunogen | Raf1 (Phospho-Ser259) antibody was raised against a peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1. |
| Modification | p-Ser259 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Alternative Names | NS5 CRAF Raf-1 c-Raf BBC6 BCL2L8 C-RAF C-Raf |
| Molecular Weight(MW) | 73 kDa |
| Tissue Specificity | This antibody detects endogenous level of Raf-1 only when phosphorylated at serine 259. |
| Entrez Gene | 5894 |
|---|---|
| Protein Accession | NP_002871.1 |
Application
Western blot analysis of lysed extracts from 293 and MDA cells untreated or treated with PMA using Raf1 (Phospho-Ser259).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Raf1 (Phospho-Ser259).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
