Application
Immunohistochemistry staining of RAF1 in kidney tissue using RAF1 Antibody.| Product Name | RAF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RAF, a member of the Raf Subfamily of Serine/Threonine Protein Kinases, is part of the RAS-dependent signalling pathway. Once activated, Raf-1 can activate the dual specificity protein kinases MEK1 and MEK2 which in turn activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. t(3;8)(p25;q21) translocations involving RAF1 result in mixed parotid gland tumors. Raf1 is an ocogene that has been reported to be overexpressed in many tumor types. |
| Immunogen | RAF1 antibody was raised against amino acids surrounding Ser 259 of RAF1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| RAF1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 1:200. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Alternative Names | RAF1 C-Raf C-raf-1 CRAF NS5 Raf-1 Proto-oncogene c-RAF Oncogene RAF1 RAF Raf1 kinase |
| Tissue Specificity | pSer259 |
Application
Immunohistochemistry staining of RAF1 in kidney tissue using RAF1 Antibody.| Application Notes | RAF1 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:2000, and immunohistochemistry starting at 1:200. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | RAF1 antibody should be stored long term (months) at -80 ˚C and short term (days) at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide, 50% glycerol. |
