Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with PMA for the indicated times using Raf1 (Phospho-Ser338).| Product Name | Raf1 (phospho Ser338) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.1) Adams DG, et al. (2005) J Biol Chem. 280 (52): 42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306 (4): 924-9. Noser JA, et al. (2007) Mol Ther. 15 (8): 1531-6. |
| Immunogen | Raf1 (Phospho-Ser338) antibody was raised against a peptide sequence around phosphorylation site of serine 338 (R-D-S (p) -S-Y) derived from Human RAF. |
| Modification | p-Ser338 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Alternative Names | NS5 CRAF Raf-1 c-Raf JAK1A JAK1B C-RAF C-Raf |
| Molecular Weight(MW) | 73 kDa |
| Tissue Specificity | This antibody detects endogenous level of Raf1 only when phosphorylated at serine 338. |
| Entrez Gene | 5894 |
|---|---|
| Protein Accession | NP_002871.1 |
Application
Western blot analysis of lysed extracts from MDA cells untreated or treated with PMA for the indicated times using Raf1 (Phospho-Ser338).| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
