Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | RUNX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RUNX1, AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1 |
| Product description | Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. RUNX1 is the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with severaltypes of leukemia.1) Ghozi, M.C., et al., (1996) Proc. Natl. Acad. Sci. U.S.A. 93 (5), 1935-1940. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| RUNX1 antibody can be used for detection of RUNX1 by ELISA at 1:12500. RUNX1 antibody can be used for detection of RUNX1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUNX1 |
|---|---|
| Alternative Names | RUNX1 AML1 CBFA2 EVI-1 AMLCR1 PEBP2aB AML1-EVI-1 |
| Molecular Weight(MW) | 12 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | RUNX1 antibody can be used for detection of RUNX1 by ELISA at 1:12500. RUNX1 antibody can be used for detection of RUNX1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store RUNX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
