Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AP3289g) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AP3289g)Application
Western blot analysis of RPS6KB1 using rabbit polyclonal RPS6KB1 Antibody (S424) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2). )WB
(a–c) ECA109 and KYSE150 cells were treated each day with 5 μM GO-203 or CP-2 for 3 days, and GO-203 inhibits the phosphorylation of AKT and S6K1 protein and then increases PDCD4 leave in ESCC cells.| Product Name | S6K Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | RPS6KB1 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding the gene for RPS6KB1 and overexpression of this kinase are seen in some breast cancer cell lines.1) Adem, C., et al., Genes Chromosomes Cancer 41(1):1-11 (2004). |
| Immunogen | This S6K (RPS6KB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from human S6K (RPS6KB1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB1 |
|---|---|
| Alternative Names | Ribosomal protein S6 kinase beta-1 S6K-beta-1 S6K1 70 kDa ribosomal protein S6 kinase 1 P70S6K1 p70-S6K 1 Ribosomal protein S6 kinase I Serine/threonine-protein kinase 14A p70 ribosomal S6 kinase alpha p70 S6 kinase alpha p70 S6K-alpha p70 S6KA |
| Molecular Weight(MW) | 59 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with RPS6KB1 Antibody (T421) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of Phospho-RPS6KB1-pS424 Antibody (AP3352a) and RPS6KB1 Antibody (S424) (AP3289g) in human TNF alpha activated Hela cell line lysates. RPS6KB1 and Phospho-RPS6KB1 was detected using the purified PAb. (lane 1: without TNF alpha; lane 2: TNF alpha activated, Phospho-RPS6KB1-pS424 Antibody (AP3352a); lane 3: TNF alpha activated, RPS6KB1 Antibody (S424) (AP3289g)
Application
Western blot analysis of RPS6KB1 using rabbit polyclonal RPS6KB1 Antibody (S424) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KB1 gene (Lane 2). )
WB
(a–c) ECA109 and KYSE150 cells were treated each day with 5 μM GO-203 or CP-2 for 3 days, and GO-203 inhibits the phosphorylation of AKT and S6K1 protein and then increases PDCD4 leave in ESCC cells.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
