Application
Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 μg/mL.Application
Western blot analysis of SAPAP1 in rat brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 μg/mL.| Product Name | SAPAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SAPAP1 Antibody: CNF, NPHN, nephrin, Nephrin, Renal glomerulus-specific cell adhesion receptor |
| Product description | SAPAP1 Antibody: SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM).1) SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem.1997; 272:11943-51. |
| Immunogen | SAPAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human SAPAP1. The immunogen is located within amino acids 640 - 690 of SAPAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SAPAP1 antibody can be used for detection of SAPAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPHS1 |
|---|---|
| Alternative Names | SAPAP1 Antibody: CNF NPHN nephrin Nephrin Renal glomerulus-specific cell adhesion receptor |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins. |
Application
Immunohistochemistry of SAPAP1 in human brain with SAPAP1 antibody at 5 μg/mL.
Application
Western blot analysis of SAPAP1 in rat brain tissue lysate with SAPAP1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of SAPAP1 in Human Brain cells with SAPAP1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SAPAP1 antibody can be used for detection of SAPAP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SAPAP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SAPAP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
