Application
Antibody used in WB on Human SW620 at 0.2-1 ug/ml.| Product Name | SCN5A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SCN5A is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. The protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram.The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. The encoded protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in two transcript variants encoding separate isoforms which differ by a single amino acid. Mutation nomenclature has been assigned with respect to the longer isoform.1) Shah, V.N., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (10), 3592-3597. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SCN5A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| SCN5A antibody can be used for detection of SCN5A by ELISA at 1:312500. SCN5A antibody can be used for detection of SCN5A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SCN5A |
|---|---|
| Alternative Names | SCN5A CDCD2 CMD1E CMPD2 HB1 HB2 HH1 IVF LQT3 Nav1.5 SSS1 VF1 HBBD ICCD PFHB1 |
| Molecular Weight(MW) | 222 kDa, 227 kDa, 36 kDa |
Application
Antibody used in WB on Human SW620 at 0.2-1 ug/ml.| Positive Control | 1) SW620 Cell Lysate using SCN5A antibody at 0.25 μg/mL (8% gel). |
|---|---|
| Application Notes | SCN5A antibody can be used for detection of SCN5A by ELISA at 1:312500. SCN5A antibody can be used for detection of SCN5A by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SCN5A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
