WB
Western blot analysis of lysates from Mouse heart using Nav1.5/SCN5A Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.| Product Name | Nav1.5/SCN5A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:5000 IHC:1:200-1:800 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | SCN5A |
|---|---|
| Gene Synonyms | HB1 HB2 HH1 IVF VF1 HBBD ICCD LQT3 SSS1 CDCD2 CMD1E CMPD2 PFHB1 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 5 |
| Gene Summary | Nav1.5 is encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. The encoded protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in two transcript variants encoding separate isoforms which differ by a single amino acid. Mutation nomenclature has been assigned with respect to the longer isoform. This antibody is expected to recognise both reported isoforms |
| Alternative Names | HB1 HB2 HH1 IVF VF1 HBBD ICCD LQT3 SSS1 CDCD2 CMD1E CMPD2 PFHB1 Nav1.5 |
| Molecular Weight(MW) | 250kDa |
| Cellular Localization | endoplasmic reticulum, lateral plasma membrane, nucleolus, nucleoplasm, perinuclear region of cytoplasm, plasma membrane, sarcolemma, T-tubule, Z disc. |
| SwissProt ID | Q14524 |
|---|
WB
Western blot analysis of lysates from Mouse heart using Nav1.5/SCN5A Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris EDTA Buffer (pH 9.0) prior to IHC staining.| Application Notes | WB:1:2000-1:5000 IHC:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
