WB
rabbit heart tissue were subjected to SDS PAGE followed by western blot with SCN5A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
rat heart tissue were subjected to SDS PAGE followed by western blot with SCN5A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using SCN5A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using SCN5A antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | SCN5A Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SCN5A protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCIF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:50-1:500 IF-P:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRabbitRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN5A |
|---|---|
| Gene Synonyms | HB1 HB2 HH1 IVF VF1 HBBD ICCD LQT3 SSS1 CDCD2 CMD1E CMPD2 PFHB1 Nav1.5 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 5 |
| Gene Summary | The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene have been associated with long QT syndrome type 3 (LQT3), atrial fibrillation, cardiomyopathy, and Brugada syndrome 1, all autosomal dominant cardiac diseases. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, May 2022] |
| Alternative Names | Nav1.5 1C2B3 CDCD2 CMD1E CMPD2 |
| Molecular Weight(MW) | 227 kDa |
| Cellular Localization | Cell junction,Cell membrane,Cytoplasm,Membrane |
WB
rabbit heart tissue were subjected to SDS PAGE followed by western blot with SCN5A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
rat heart tissue were subjected to SDS PAGE followed by western blot with SCN5A antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using SCN5A antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using SCN5A antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN5A and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | WB:1:2000-1:10000 IHC:1:50-1:500 IF-P:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
