WB
Western blot analysis of lysates from SW620 cells using Nav1.5/SCN5A Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.IHC
Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:200 .| Product Name | Nav1.5/SCN5A Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:200 |
|
| Species Reactivity | Human |
| Concentration | 1μg/μl |
| Purification | Protein A/G |
| Gene Symbol | SCN5A |
|---|---|
| Gene Synonyms | HB1 HB2 HH1 IVF VF1 HBBD ICCD LQT3 SSS1 CDCD2 CMD1E CMPD2 PFHB1 |
| Gene Summary | Nav1.5 is encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. The encoded protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in two transcript variants encoding separate isoforms which differ by a single amino acid. Mutation nomenclature has been assigned with respect to the longer isoform. This antibody is expected to recognise both reported isoforms |
| Molecular Weight(MW) | 250kDa |
| SwissProt ID | Q14524 |
|---|
WB
Western blot analysis of lysates from SW620 cells using Nav1.5/SCN5A Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human heart tissue using Nav1.5/SCN5A Rabbit mAb at a dilution of 1:200 .| Application Notes | WB:1:500-1:1000 IHC:1:200 |
|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
|---|---|
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol, pH 7.2 |
