Western blot
HEK cel lysate; HEK cel with HuNav1.5 transfected lysate; Mouse Heart lysate80 ug protein on gelQC Antibodies 1:100 in PBS/Tweensecondary antibodie 1:10.000 ( IRDYE 800 CWGoat anti Rabbit IgG) analyse with the Licor OdysseyPositive Control: HEK cel lysate; HEK cel with HuNav1.5 transfected lysate; Mouse Heart lysate80 ug protein on gelQC Antibodies 1:100 in PBS/Tweensecondary antibodie 1:10.000 ( IRDYE 800 CWGoat anti Rabbit IgG) analyse with the Licor Odyssey