Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 (Phospho-Ser80). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (Phospho-Ser80).Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).| Product Name | SEK1 / MKK4 (phospho Ser80) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).1) Park HS, et al. (2002) J Biol Chem; 277 (4): 2573-8. |
| Immunogen | SEK1/MKK4 (Phospho-Ser80) antibody was raised against a peptide sequence around phosphorylation site of serine 80 (T-H-S (p) -I-E) derived from Human SEK1/MKK4. |
| Modification | p-Ser80 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP2K4 |
|---|---|
| Alternative Names | JNKK MEK4 MKK4 SEK1 SKK1 JNKK1 SERK1 MAPKK4 PRKMK4 SAPKK1 SAPKK-1 SRK JNKK MAP2K4 MAPK/ERK kinase 4 MAPKK 4 |
| Molecular Weight(MW) | 44 kDa |
| Tissue Specificity | This antibody detects endogenous level of SEK1/MKK4 only when phosphorylated at serine 80. |
| Entrez Gene | 6416 |
|---|---|
| Protein Accession | NP_003001.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4 (Phospho-Ser80). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using SEK1/MKK4 (Phospho-Ser80).
Application
Western blot analysis of lysed extracts from HepG2 cells untreated or treated with EGF using SEK1/MKK4 (Phospho-Ser80).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
