Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.| Product Name | TECK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Thymus expressed chemokine, Small inducible cytokine A25, CCL25, SCYA25, TECK, C-C motif chemokine 25, Chemokine TECKTECK, Ckb15 |
| Product description | This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified. |
| Immunogen | The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTECK as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCL25 |
|---|---|
| Alternative Names | Thymus expressed chemokine Small inducible cytokine A25 CCL25 SCYA25 TECK C-C motif chemokine 25 Chemokine TECKTECK Ckb15 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 2.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen and a Proteinase K antigen retrieval. Optimal concentrations and conditions may vary.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTECK (100 ng/mL), a concentration of 10.0-20.0 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human TECK when used with our biotinylated antigen affinity purified anti-human TECK (XP-5280Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 0.50-1.0 ng/lane, under non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | TECK lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
