Application
Western blot analysis of TLR4 in human small intestine lysate with TLR4 antibody at (A) 2 and (B) 4 μg/mL.Application
Immunohistochemistry of TLR4 in human spleen tissue with TLR4 antibody at 2.5 μg/ml.Application
Immunofluorescence of TLR4 in human spleen tissue with TLR4 antibody at 20 μg/ml.Application
Immunocytochemistry of TLR4 in K562 cells with TLR4 antibody at 2 μg/mL.| Product Name | TLR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR4 Antibody: TOLL, CD284, TLR-4, ARMD10, Toll-like receptor 4, hToll |
| Product description | TLR4 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.1) Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. |
| Immunogen | TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4. The immunogen is located within amino acids 30 - 80 of TLR4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TLR4 |
|---|---|
| Alternative Names | TLR4 Antibody: TOLL CD284 TLR-4 ARMD10 Toll-like receptor 4 hToll |
| Molecular Weight(MW) | Predicted: 92 kDa Observed: 94 kDa |
| Sequence Similarities | 3) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of TLR4 in human small intestine lysate with TLR4 antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunohistochemistry of TLR4 in human spleen tissue with TLR4 antibody at 2.5 μg/ml.
Application
Immunofluorescence of TLR4 in human spleen tissue with TLR4 antibody at 20 μg/ml.
Application
Immunocytochemistry of TLR4 in K562 cells with TLR4 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | TLR4 antibody can be used for detection of TLR4 by Western blot at 2 to 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TLR4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TLR4 Antibody is supplied in PBS containing 0.02% sodium azide. |
