Application
Immunohistochemistry staining of TLR4 in breast tissue using TLR4 monoclonal Antibody.| Product Name | TLR4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll |
| Product description | The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88 kDa. Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis. |
| Immunogen | TLR4 monoclonal antibody was raised against a portion of amino acids 100 - 200 of TLR4 (Human). |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | FACSIHCWB |
| TLR4 antibody can be used in immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | TLR4 |
|---|---|
| Alternative Names | TLR4 CD284 CD284 antigen Homolog of Drosophila toll TLR-4 TOLL ARMD10 Toll-like receptor 4 HToll |
| Tissue Specificity | The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse. |
Application
Immunohistochemistry staining of TLR4 in breast tissue using TLR4 monoclonal Antibody.| Application Notes | TLR4 antibody can be used in immunohistochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | TLR4 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
