Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | TNFSF10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TNFSF10, TL2, APO2L, CD253, TRAIL, Apo-2L |
| Product description | TNFSF10 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.1) Wu, Y.Y., et al., (2004) World J. Gastroenterol. 10 (16), 2334-2339. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TNFSF10. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TNFSF10 antibody can be used for detection of TNFSF10 by ELISA at 1:62500. TNFSF10 antibody can be used for detection of TNFSF10 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF10 |
|---|---|
| Alternative Names | TNFSF10 TL2 APO2L CD253 TRAIL Apo-2L |
| Molecular Weight(MW) | 33 kDa |
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TNFSF10 antibody can be used for detection of TNFSF10 by ELISA at 1:62500. TNFSF10 antibody can be used for detection of TNFSF10 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TNFSF10 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
