Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vaApplication
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vaApplication
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va| Product Name | TGFb1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2, CD253 |
| Immunogen | The monoclonal antibody was produced from a hybridoma using purified recombinant, insect cell derived, hTGFβ1. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. Western Blot: To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF10 |
|---|---|
| Alternative Names | APO2L TRAIL Tumor necrosis factor ligand superfamily member 10 Apo-2 ligand Apo-2L TL2 CD253 |
Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va
Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va
Application
This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may va| Application Notes | ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human TGFβ1 when used in conjunction with compatible secondary reagents. Western Blot: To detect hTGFβ1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGFβ1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with human TGFβ2 and TGFβ3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | TGFb1 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
