Application
This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 ug/ml- 1.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary.| Product Name | TRAIL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APO2L, Tumor Necrosis Factor (ligand) Superfamily Member 10, CD253, APO2L, TRAIL, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, Apo-2L, TL2 |
| Immunogen | The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTRAIL/APO-II as the immunizing antigen. |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF10 |
|---|---|
| Alternative Names | APO2L Tumor Necrosis Factor (ligand) Superfamily Member 10 CD253 APO2L TRAIL Tumor necrosis factor ligand superfamily member 10 Apo-2 ligand Apo-2L TL2 |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 ug/ml- 1.5 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL. Western Blot: To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Trail/Apo2L lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
