Application
Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 μg/mL.Application
Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 μg/mL.Application
Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 μg/mL.| Product Name | Trail Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Trail Antibody: TL2, APO2L, CD253, TRAIL, Apo-2L, Tumor necrosis factor ligand superfamily member 10, Apo-2 ligand |
| Product description | Trail Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. TRAIL (TNF-related apoptosis-inducing ligand) is a type II membrane protein and expressed in a variety of human tissues (1,2). The death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL (3-6). Like TNF and Fas ligand, TRAIL induces apoptosis and NF-κB activation in many tissues and cells.1) Wiley SR, Schooley K, Smolak PJ, et al. Identification and characterization of a new member of the TNF family that induces apoptosis. Immunity 1995; 3:673-82. |
| Immunogen | TRAIL antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TRAIL. The immunogen is located within the last 50 amino acids of Trail. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TRAIL antibody can be used for detection of TRAIL by Western blot at 1 - 5 μg/mL dilution. Antibody can also be used for immunohistochemistry starting at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF10 |
|---|---|
| Alternative Names | Trail Antibody: TL2 APO2L CD253 TRAIL Apo-2L Tumor necrosis factor ligand superfamily member 10 Apo-2 ligand |
| Molecular Weight(MW) | Predicted: 32 kDa Observed: 35 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of TRAIL in HeLa cell lysate containing 10, 2.5, or 1 ng of recombinant protein containing extracellular domain of TRAIL with TRAIL antibody at 1 μg/mL.
Application
Western blot analysis of TRAIL in human brain tissue lysate with TRAIL antibody at (A) 2.5 and (B) 5 μg/mL.
Application
Immunohistochemistry of TRAIL in human brain tissue with TRAIL antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TRAIL antibody can be used for detection of TRAIL by Western blot at 1 - 5 μg/mL dilution. Antibody can also be used for immunohistochemistry starting at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Trail antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Trail Antibody is supplied in PBS containing 0.02% sodium azide. |
