Application
Western blot analysis of lysate from LNCaP cell line,using TRPM8 Antibody at 1:1000 at each laneApplication
TRPM8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of TRPM8 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | TRPM8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.1) Yee, N.S., et al. Cancer Lett. 297(1):49-55(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Kuhn, F.J., et al. J. Biol. Chem. 285(35):26806-26814(2010) Gkika, D., et al. Oncogene 29(32):4611-4616(2010) Van Haute, C., et al. ScientificWorldJournal 10, 1597-1611 (2010) : |
| Immunogen | This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the Central region of human TRPM8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | TRPM8 |
|---|---|
| Alternative Names | Transient receptor potential cation channel subfamily M member 8 Long transient receptor potential channel 6 LTrpC-6 LTrpC6 Transient receptor potential p8 Trp-p8 TRPM8 LTRPC6 TRPP8 |
| Molecular Weight(MW) | 128 kDa |
Application
Western blot analysis of lysate from LNCaP cell line,using TRPM8 Antibody at 1:1000 at each lane
Application
TRPM8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of TRPM8 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
