Application
Western blot analysis of TRPM8 using rabbit polyclonal TRPM8 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | TRPM8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | TRPM8 is a receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. It is activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH and involved in menthol sensation. It is permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.1) Tsavaler L.,Cancer Res. 61:3760-3769(2001). Kiessling A.,Prostate 56:270-279(2003). Bodding,M.,Cell Calcium 42 (6), 618-628 (2007) |
| Immunogen | This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1075-1104 amino acids from the C-terminal region of human TRPM8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | TRPM8 |
|---|---|
| Alternative Names | Transient receptor potential cation channel subfamily M member 8 Long transient receptor potential channel 6 LTrpC-6 LTrpC6 Transient receptor potential p8 Trp-p8 TRPM8 LTRPC6 TRPP8 |
| Molecular Weight(MW) | 128 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of TRPM8 using rabbit polyclonal TRPM8 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TRPM8 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TRPM8 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
