Application
Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)Application
ZO1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ZO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1, ZO1 |
| Product description | ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions.1) Ohira, M., et al. Int. J. Mol. Med. 24(6):829-835(2009) |
| Immunogen | This ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1570-1600 amino acids from the C-terminal region of human ZO1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | pig,cow,Guinea Pig |
| Concentration | 1mg/ml |
| Gene Symbol | TJP1 |
|---|---|
| Alternative Names | Tight junction protein ZO-1 Tight junction protein 1 Zona occludens protein 1 Zonula occludens protein 1 TJP1 ZO1 |
| Molecular Weight(MW) | 195 kDa |
Application
Western blot analysis of ZO1 Antibody in K562 cell line lysates (35ug/lane)
Application
ZO1 Antibody IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
