WB
Western blot analysis of extracts from UV treated RAW264.7 cells, using Phospho-STING (Ser366) Ab. The lane on the left was treated with blocking peptide.IHC
Phospho-STING (Ser366) Ab at 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.ICC/IF
Phospho-STING (Ser366) staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue)| Product Name | Phospho-TMEM173/STING (Ser366) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human TMEM173/STING around the phosphorylation site of Ser366. |
| Modification | p-Ser366 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 IF/ICC:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Purification | Affinity purified |
| Gene Symbol | STING1 |
|---|---|
| Gene Synonyms | ERIS MITA MPYS SAVI NET23 STING hMITA hSTING TMEM173 STING-beta |
| Molecular Weight(MW) | 35-40kD |
WB
Western blot analysis of extracts from UV treated RAW264.7 cells, using Phospho-STING (Ser366) Ab. The lane on the left was treated with blocking peptide.
IHC
Phospho-STING (Ser366) Ab at 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.
ICC/IF
Phospho-STING (Ser366) staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue)| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 IF/ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
