WB
Cerebrum (Mouse) Lysate at 40 ug Stomach (Mouse) Lysate at 40 ug Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionIHC
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.| Product Name | Phospho-Smad1/5 (Ser463 + Ser465) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human Smad1/5 around the phosphorylation site of Ser463/465: IS(p-S)V(p-S) |
| Modification | p-Ser463 + Ser465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 ICC:1:100-1:500 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Smad1/5 |
|---|---|
| Gene Synonyms | BSP1 JV41 BSP-1 JV4-1 MADH1 MADR1 |
| Gene Full Name | SMAD family member 1/5 |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Alternative Names | BSP-1 BSP1 JV4-1 JV41 MADH1 MADR1 Mad1 Mlp1 MusMLP dwf-A mMad1 SMAD1_HUMAN SMAD1 MAD homolog 1 Mothers against DPP homolog 1 Mad-related protein 1 SMAD family member 1 (SMAD 1 | Smad1 | hSMAD1) Transforming growth factor-beta-signaling protein 1 (BSP-1) SMAD1_MOUSE Dwarfin-A (Dwf-A) Mothers-against-DPP-related 1 (Mad-related protein 1 | mMad1) SMAD family member 1 (SMAD 1 | Smad1) SMAD1_RAT SMAD family member 1 MAD mothers against decapentaplegic homolog 1 (Drosophila) SMAD mothers against DPP homolog 1 (Drosophila) SMAD1 & SMAD5 (phospho-S463/S465) p-SMAD1 & SMAD5 phospho-SMAD1 & SMAD5 SMAD1 & SMAD5 (phospho-Ser463/Ser465) |
| Molecular Weight(MW) | 52kDa(Observed band size: 62 kDa) |
| Function | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. |
| Tissue Specificity | Ubiquitous. Highest expression seen in the heart and skeletal muscle. |
| Cellular Localization | ytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. |
WB
Cerebrum (Mouse) Lysate at 40 ug Stomach (Mouse) Lysate at 40 ug Primary: Anti-Phospho-Smad1/5 (Ser463 + Ser465) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
IHC
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Smad1 5 (Ser463 + Ser465)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 ICC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
