WB
Western blot analysis of SMAD1/5 using anti-SMAD1/5 antibody. Lane 1: rat kidney tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: mouse brain tissue lysate,Lane 4: mouse kidney tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: human Hela cell lysate,Lane 7: human A375 cell lysate,Lane 8: human HepG2 cell lysate. anti-SMAD1/5 antigen affinity purified polyclonal antibody probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for SMAD1/5 at approximately 52,60KD. The expected band size for SMAD1/5 is at 48, 52, 60KD.| Product Name | Anti-SMAD1/5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human SMAD1/SMAD5, identical to the related mouse and rat sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 500/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD1/SMAD5 |
|---|---|
| Gene Synonyms | BSP1 JV41 BSP-1 JV4-1 MADH1 MADR1 |
| Gene Full Name | SMAD family member 1/5 |
| Gene Summary | The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 48, 52, 60 kDa |
WB
Western blot analysis of SMAD1/5 using anti-SMAD1/5 antibody. Lane 1: rat kidney tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: mouse brain tissue lysate,Lane 4: mouse kidney tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: human Hela cell lysate,Lane 7: human A375 cell lysate,Lane 8: human HepG2 cell lysate. anti-SMAD1/5 antigen affinity purified polyclonal antibody probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for SMAD1/5 at approximately 52,60KD. The expected band size for SMAD1/5 is at 48, 52, 60KD.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
