WB
Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: HEK-293 cell lysate (30 µg/Lane) Lane 4: A431 cell lysate (30 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Adipose Triglyceride Lipase Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Adipose Triglyceride Lipase aa 330-450 / 504. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000 IHC:1:500-1:2000 IF-P:1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PNPLA2 |
|---|---|
| Gene Synonyms | ATGL TTS2 PEDF-R FP17548 TTS-2.2 iPLA2zeta 1110001C14Rik |
| Gene Full Name | patatin like phospholipase domain containing 2 |
| Gene Summary | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
| Alternative Names | 1110001C14Rik antibody Adipose triglyceride lipase antibody ATGL antibody ATGL DESNUTRIN antibody Calcium independent phospholipase A2 antibody Calcium-independent phospholipase A2 antibody Desnutrin antibody EC 3.1.1.3 antibody FP17548 antibody IPLA2 zeta antibody 1110001C14Rik antibody Adipose triglyceride lipase antibody ATGL antibody ATGL DESNUTRIN antibody Calcium independent phospholipase A2 antibody Calcium-independent phospholipase A2 antibody Desnutrin antibody EC 3.1.1.3 antibody FP17548 antibody IPLA2 zeta antibody IPLA2-zeta antibody Mutant patatin like phospholipase domain containing 2 antibody Patatin like phospholipase domain containing 2 antibody PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2 antibody Patatin-like phospholipase domain-containing protein 2 antibody PEDF R antibody PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA antibody Pigment epithelium derived factor antibody Pigment epithelium-derived factor antibody plpl antibody plpl2 antibody PLPL2_HUMAN antibody Pnpla2 antibody Transport secretion protein 2 antibody Transport secretion protein 2.2 antibody Transport-secretion protein 2 antibody Triglyceride hydrolase antibody TTS 2.2 antibody TTS2 antibody TTS2.2 antibody ZETA antibody |
| Molecular Weight(MW) | 55kDa |
| Cellular Localization | Lipid droplet, Cell membrane, Cytoplasm. |
WB
Western blot analysis of Adipose Triglyceride Lipase on different lysates with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/1,000 dilution. Lane 1: SiHa cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: HEK-293 cell lysate (30 µg/Lane) Lane 4: A431 cell lysate (30 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat heart tissue lysate (40 µg/Lane) Exposure time: Lane 1-4: 3 minutes; Lane 5-6: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of paraffin-embedded mouse brown adipose tissue labeling Adipose Triglyceride Lipase with Rabbit anti-Adipose Triglyceride Lipase antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:1000 IHC:1:500-1:2000 IF-P:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
