WB
Western blot analysis of various lysates using NG2/CSPG4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): HL-60 Exposure time: 10s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma tissue using NG2/CSPG4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.ICC/IF
Confocal imaging of SK-MEL-28 cells using NG2/CSPG4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). objective: 100x.FC
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and SK-MEL-28 cells (right) were surface-stained with NG2/CSPG4 Rabbit mAb (2 μg/mL,orange line) or Alexa Fluor® 647 Rabbit IgG isotype control (5 μl/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line)| Product Name | NG2/CSPG4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1583-2224 of human NG2 (NP_001888.2). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000-1:4000 IHC:1:200-1:800 ICC:1:200-1:800 FC:1:500-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSPG4 |
|---|---|
| Gene Synonyms | NG2 MCSP MCSPG MSK16 CSPG4A HMW-MAA MEL-CSPG |
| Gene Full Name | chondroitin sulfate proteoglycan 4 |
| Gene Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008] |
| Alternative Names | NG2 MCSP MCSPG MSK16 CSPG4A HMW-MAA MEL-CSPG NG2/CSPG4 |
| Molecular Weight(MW) | 251kDa(Observed MW 330kDa) |
| Cellular Localization | Apical cell membrane,Cell projection,Extracellular side,Single-pass type I membrane protein,lamellipodium membrane. |
WB
Western blot analysis of various lysates using NG2/CSPG4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): HL-60 Exposure time: 10s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma tissue using NG2/CSPG4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ICC/IF
Confocal imaging of SK-MEL-28 cells using NG2/CSPG4 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). objective: 100x.
FC
Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and SK-MEL-28 cells (right) were surface-stained with NG2/CSPG4 Rabbit mAb (2 μg/mL,orange line) or Alexa Fluor® 647 Rabbit IgG isotype control (5 μl/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line)| Application Notes | WB:1:1000-1:4000 IHC:1:200-1:800 ICC:1:200-1:800 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
