WB
Western blot analysis of lysates from U-138 MG cells, using NG2/CSPG4 Rabbit pAb at 1:6000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.ICC/IF
Immunofluorescence analysis of A-375 cellsusing NG2/CSPG4 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using NG2/CSPG4Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M Tris/EDTA Buffer (pH9.0) prior to IHC staining.| Product Name | NG2/CSPG4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:7000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSPG4 |
|---|---|
| Gene Synonyms | NG2 MCSP MCSPG MSK16 CSPG4A HMW-MAA MEL-CSPG |
| Gene Full Name | chondroitin sulfate proteoglycan 4 |
| Gene Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 251kDa(Observed band size:250-450kDa) |
| Cellular Localization | Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane. |
WB
Western blot analysis of lysates from U-138 MG cells, using NG2/CSPG4 Rabbit pAb at 1:6000dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.
ICC/IF
Immunofluorescence analysis of A-375 cellsusing NG2/CSPG4 Rabbit pAb at dilution of 1:100 (40x lens). Secondaryantibody: Cy3-conjugated Goat anti-RabbitIgG (H+L) at 1:500 dilution. Blue:DAPI for nuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse brain using NG2/CSPG4Rabbit pAb at dilution of 1:100(40x lens). Microwave antigen retrievalperformed with 0.01M Tris/EDTA Buffer (pH9.0) prior to IHC staining.| Application Notes | WB:1:2000-1:7000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
