WB
Relative expression (RE). Western blot analysis of NG2/CSPG4 on different lysates with Rabbit anti-NG2/CSPG4 antibody at 1/10,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 6 seconds; Lane 3-6: 59 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Relative expression (RE). Immunocytochemistry analysis of A375 (positive) and SK-Br-3 (negative) labeling NG2/CSPG4 with Rabbit anti-NG2/CSPG4 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NG2/CSPG4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Relative expression (RE). Flow cytometric analysis of SK-Br-3 (left, negative) and A375 (right, positive) cells labeling NG2/CSPG4. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | NG2/CSPG4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human NG2 aa 1,301-2,224. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:10000 ICC/IF:1:100 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CSPG4 |
|---|---|
| Gene Synonyms | NG2 MCSP MCSPG MSK16 CSPG4A HMW-MAA MEL-CSPG |
| Gene Full Name | chondroitin sulfate proteoglycan 4 |
| Gene Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008] |
| Alternative Names | 4732461B14Rik antibody AN2 antibody AN2 proteoglycan antibody Chondroitin sulfate proteoglycan 4 (melanoma-associated) antibody Chondroitin sulfate proteoglycan 4 antibody Chondroitin sulfate proteoglycan NG2 antibody Cspg4 antibody Cspg4 chondroitin sulfate proteoglycan 4 antibody CSPG4_HUMAN antibody HMW-MAA antibody HSN tumor-specific antigen antibody Kiaa4232 antibody MCSP antibody MCSPG antibody MEL-CSPG antibody Melanoma chondroitin sulfate proteoglycan antibody Melanoma-associated chondroitin sulfate proteoglycan antibody MELCSPG antibody MSK16 antibody NG2 antibody |
| Molecular Weight(MW) | 251 kDa(Observed band size:330 kDa) |
| Cellular Localization | Cell membrane, Apical cell membrane, Cell projection, lamellipodium membrane, Cell surface. |
WB
Relative expression (RE). Western blot analysis of NG2/CSPG4 on different lysates with Rabbit anti-NG2/CSPG4 antibody at 1/10,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-2: 6 seconds; Lane 3-6: 59 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Relative expression (RE). Immunocytochemistry analysis of A375 (positive) and SK-Br-3 (negative) labeling NG2/CSPG4 with Rabbit anti-NG2/CSPG4 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NG2/CSPG4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Relative expression (RE). Flow cytometric analysis of SK-Br-3 (left, negative) and A375 (right, positive) cells labeling NG2/CSPG4. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:10000 ICC/IF:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
