IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody at dilution of 1:100.FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (red), or 0.4 ug . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer .| Product Name | CoraLite®594-conjugated Bcl-XL Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. |
| Conjugation | Agarose |
| Clonality | Monoclonal |
|---|---|
| Isotype | Ig2b |
| Host Species | Mouse |
| Tested Applications | FCICC/IF |
| FC:1:50~1:500: ICC:1:50-1:200 |
|
| Species Reactivity | CanineHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Bcl-XL |
|---|---|
| Gene Synonyms | BCL-XL/S BCL2L BCLX Bcl-X PPP1R52 |
| Gene Full Name | BCL2L1 BCL2 like 1 [ Homo sapiens (human) ] |
| Gene Summary | The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015] |
| Alternative Names | Bcl-xL 4C12A6 Apoptosis regulator Bcl X Apoptosis regulator Bcl-X Bcl2-L-1 |
| Molecular Weight(MW) | 26 |
IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-XL antibody at dilution of 1:100.
FC
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Bcl-XL (red), or 0.4 ug . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer .| Application Notes | FC:1:50~1:500: ICC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Buffer | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
