WB
Western blot analysis of Prolactin Receptor on different lysates with Mouse anti-Prolactin Receptor antibody at 1/500 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Prolactin Receptor antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of MCF-7 cells labeling Prolactin Receptor with Mouse anti-Prolactin Receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Prolactin Receptor antibody at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of HeLa cells labeling Prolactin Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Prolactin Receptor Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human Prolactin Receptor aa 150-350 / 622. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500 IHC:1:200 ICC:1:100 FC:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRLR |
|---|---|
| Gene Synonyms | HPRL MFAB hPRLrI RI-PRLR |
| Gene Full Name | prolactin receptor |
| Gene Summary | This gene encodes a receptor for the anterior pituitary hormone, prolactin, and belongs to the type I cytokine receptor family. Prolactin-dependent signaling occurs as the result of ligand-induced dimerization of the prolactin receptor. Several alternatively spliced transcript variants encoding different membrane-bound and soluble isoforms have been described for this gene, which may function to modulate the endocrine and autocrine effects of prolactin in normal tissue and cancer. [provided by RefSeq, Feb 2011] |
| Alternative Names | AI987712 antibody CLONE SPM213 antibody CPRLP antibody Delta 4-delta 7/11 truncated prolactin receptor antibody Delta 4-SF1b truncated prolactin receptor antibody HPRL antibody hPRL receptor antibody hPRLrI antibody Lactogen receptor antibody MFAB antibody AI987712 antibody CLONE SPM213 antibody CPRLP antibody Delta 4-delta 7/11 truncated prolactin receptor antibody Delta 4-SF1b truncated prolactin receptor antibody HPRL antibody hPRL receptor antibody hPRLrI antibody Lactogen receptor antibody MFAB antibody MGC105486 antibody OPR antibody OTTHUMP00000115998 antibody Pr-1 antibody Pr-3 antibody PRL R antibody PRL-R antibody PRLR antibody Prlr-rs1 antibody PRLR_HUMAN antibody Prolactin receptor a antibody Prolactin receptor antibody Prolactin receptor delta 7/11 antibody RATPRLR antibody Secreted prolactin binding protein antibody Truncated testis-specific box 1-C prolactin receptor antibody wu:fj65c07 antibody |
| Molecular Weight(MW) | 70kDa(Observed band size: 100kDa) |
| Cellular Localization | Membrane, Secreted. |
WB
Western blot analysis of Prolactin Receptor on different lysates with Mouse anti-Prolactin Receptor antibody at 1/500 dilution. Lane 1: 293T cell lysate (10 µg/Lane) Lane 2: Hela cell lysate (10 µg/Lane) Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Prolactin Receptor antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of MCF-7 cells labeling Prolactin Receptor with Mouse anti-Prolactin Receptor antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Prolactin Receptor antibody at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of HeLa cells labeling Prolactin Receptor. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:500 IHC:1:200 ICC:1:100 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
