WB
Phospho-CaMKII alpha/delta (Thr286) Antibody-Effects of cypermethrin on Ca2+/CaM/CaMKII pathway.IHC
Phospho-CaMKII alpha/delta (Thr286) Antibody at 1/100 staining human kindey carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.ICC/IF
Phospho-CaMKII alpha/delta (Thr286) Antibody staining H2O2 treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Product Name | Rabbit polyclonal antibody to Phospho-CaMKII alpha/delta (Thr286) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human CAMK2A(Accession Q9UQM7), corresponding to amino acid residues around phosphorylated Thr286. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK2A/D |
|---|---|
| Gene Synonyms | CAMKA MRD53 MRT63 CaMKIIalpha CaMKIINalpha CAMKD |
| Gene Full Name | calcium/calmodulin dependent protein kinase II alpha/delta |
| Gene Summary | Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq, Aug 2009] |
| Alternative Names | Alpha CaMKII Calcium calmodulin dependent protein kinase II Calcium/calmodulin dependent protein kinase II alpha B subunit Calcium/calmodulin dependent protein kinase type II alpha chain Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha Calcium/calmodulin-dependent protein kinase II alpha Calcium/calmodulin-dependent protein kinase II-alpha Calcium/calmodulin-dependent protein kinase type II subunit alpha Calcium/calmodulin-dependent protein kinase type IIA CaM kinase II alpha chain CaM kinase II alpha subunit CaM kinase II subunit alpha CaMK II alpha subunit CaMK-II subunit alpha Camk2a CAMKA CaMKII CaMKIINalpha EC 2.7.11.17 KCC2A_HUMAN KIAA0968 MGC123320 MGC139375 MGC155201 mKIAA0968 PK2CDD PKCCD R74975 zgc:112538 zgc:123320 Calcium / calmodulin dependent protein kinase 2 delta Calcium / calmodulin dependent protein kinase II delta calcium/calmodulin-dependent protein kinase (CaM kinase) II delta calcium/calmodulin-dependent protein kinase type II delta chain Calcium/calmodulin-dependent protein kinase type II subunit delta CAM kinase 2 delta CAM kinase II delta CaM kinase II delta subunit CaM kinase II subunit delta CaM-kinase II delta chain CAMK 2d CaMK-II delta subunit CaMK-II subunit delta CAMK2D CAMKD CAMKI KCC2D_HUMAN RATCAMKI |
| Molecular Weight(MW) | 54kDa,56kDa |
| Cellular Localization | Cell junction>Synapse. Cell junction>Synapse>Postsynaptic density. Cell projection>Dendritic spine. Cell projection>Dendrite. |
WB
Phospho-CaMKII alpha/delta (Thr286) Antibody-Effects of cypermethrin on Ca2+/CaM/CaMKII pathway.
IHC
Phospho-CaMKII alpha/delta (Thr286) Antibody at 1/100 staining human kindey carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
ICC/IF
Phospho-CaMKII alpha/delta (Thr286) Antibody staining H2O2 treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
