WB
Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500.WB
Western blot analysis of lysates from K562 cells, using CaMK2 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.ICC/IF
Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | CaMKIIα/δ (Phospho Thr286) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305. |
| Modification | P-Thr286 |
| Modification Notes | Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CAMK2A,CAMK2D |
|---|---|
| Gene Synonyms | CAMKA MRD53 MRT63 CaMKIIalpha CaMKIINalpha CAMKD |
| Gene Full Name | calcium/calmodulin dependent protein kinase II alpha/delta |
| Gene Summary | he product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2018] |
| Alternative Names | CAMK2A CAMKA KIAA0968 Calcium/calmodulin-dependent protein kinase type II subunit alpha CaM kinase II subunit alpha CaMK-II subunit alpha CAMK2D CAMKD Calcium/calmodulin-dependent protein kinase type II subunit delta CaM kinase II |
| Molecular Weight(MW) | 54kDa |
| Cellular Localization | Cell junction, synapse . Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, dendrite . Postsynaptic lipid rafts. |
WB
Western Blot analysis of HELA cells using Phospho-CaMKIIα/δ (T286) Polyclonal Antibody diluted at 1:500.
WB
Western blot analysis of lysates from K562 cells, using CaMK2 (Phospho-Thr286) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
ICC/IF
Immunofluorescence analysis of COS7 cells, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
