WB
Various lysates were subjected to SDS PAGE followed by western blot with C14orf49 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody at dilution of 1:200 and Omnimabs® 488 Goat Anti-Mouse IgG(H&L).