WB
AR/Androgen Receptor Antibody Alexa Fluor ® 790: AF790. Direct near-infrared Western blot analysis of AR expression in T-47D nuclear extract (A) and NIH/3T3 whole cell lysate (B).WB
Near infrared Western blot analysis of AR expression in whole cell lysate of AR/androgen receptor antibodies ZR-75-1 (A), MCF7 (B), and LNCaP (C).IHC/IF
AR/androgen receptor antibody Immunofluorescence staining of methanol fixed T-47D cells (A) and formalin fixed, immunoperoxidase staining of paraffin embedded human breast cancer tissue (B), showing nuclear localization.| Product Name | Mouse Monoclonal antibody to Androgen Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against amino acids 299-315 of AR of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AR |
|---|---|
| Gene Synonyms | AIS8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, AR |
| Gene Full Name | androgen receptor |
| Gene Summary | The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017] |
| Molecular Weight(MW) | 110kDa |
WB
AR/Androgen Receptor Antibody Alexa Fluor ® 790: AF790. Direct near-infrared Western blot analysis of AR expression in T-47D nuclear extract (A) and NIH/3T3 whole cell lysate (B).
WB
Near infrared Western blot analysis of AR expression in whole cell lysate of AR/androgen receptor antibodies ZR-75-1 (A), MCF7 (B), and LNCaP (C).
IHC/IF
AR/androgen receptor antibody Immunofluorescence staining of methanol fixed T-47D cells (A) and formalin fixed, immunoperoxidase staining of paraffin embedded human breast cancer tissue (B), showing nuclear localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
