WB
Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.IF-P
citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.IF-P
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4℃, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.IF-P
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4℃, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.| Product Name | Osteocalcin Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human Osteocalcin: 21-100/100. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BGLAP |
|---|---|
| Gene Synonyms | OC BGP OCN |
| Gene Full Name | bone gamma-carboxyglutamate protein |
| Gene Summary | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015] |
| Alternative Names | BGLAP antibody BGP antibody Bone gamma carboxyglutamate (gla) protein antibody Bone gamma carboxyglutamate gla protein osteocalcin antibody Bone gamma carboxyglutamate protein antibody Bone Gla protein antibody Gamma carboxyglutamic acid containing protein antibody Gamma-carboxyglutamic acid-containing protein antibody OC antibody OCN antibody BGLAP antibody BGP antibody Bone gamma carboxyglutamate (gla) protein antibody Bone gamma carboxyglutamate gla protein osteocalcin antibody Bone gamma carboxyglutamate protein antibody Bone Gla protein antibody Gamma carboxyglutamic acid containing protein antibody Gamma-carboxyglutamic acid-containing protein antibody OC antibody OCN antibody OSTCN_HUMAN antibody Osteocalcin antibody OTTHUMP00000016586 antibody PMF1 antibody |
| Molecular Weight(MW) | 11kDa |
| Cellular Localization | Secreted. |
WB
Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IF-P
citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37℃, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.
IF-P
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4℃, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
IF-P
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37℃ for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:100 overnight at 4℃, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
