IHC
IHC staining of human osteosarcoma using Osteocalcin/OCN Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using Osteocalcin antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
IHC staining of mouse lung using Osteocalcin/OCN Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using Osteocalcin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
IF Staining of NIH/3T3 using Osteocalcin/OCN Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin/OCN antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).FC
FC experiment of U2OS using Osteocalcin/OCN Polyclonal antibody. 1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Product Name | Osteocalcin/OCN Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHC |
| IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BGLAP |
|---|---|
| Gene Synonyms | OC BGP OCN |
| Gene Full Name | bone gamma-carboxyglutamate protein |
| Gene Summary | This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015] |
| Alternative Names | BGLAP Osteocalcin BGP Bone Gla protein Gamma-carboxyglutamic acid-containing protein |
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Secreted. |
IHC
IHC staining of human osteosarcoma using Osteocalcin/OCN Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using Osteocalcin antibody at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
IHC staining of mouse lung using Osteocalcin/OCN Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using Osteocalcin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
IF Staining of NIH/3T3 using Osteocalcin/OCN Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin/OCN antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC
FC experiment of U2OS using Osteocalcin/OCN Polyclonal antibody. 1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody and 488-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.| Application Notes | IHC:1:50-1:500 ICC/IF:1:200-1:800 FC:0.40 ug per 10^6 cells in a 100 µl suspension |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
