WB
Western blot analysis of CD300A using anti-CD300A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of U2OS cells using anti-CD300A antibody. Overlay histogram showing U2OS cells stained with anti-CD300A antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD300A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to CD300A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human CD300A recombinant protein (Position: L156-T299). Human CD300A shares 54.7% and 55.7% amino acid (aa) sequence identity with mouse and rat CD300A, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCWB |
| WB:1:500-1:2000 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD300A |
|---|---|
| Gene Synonyms | IRC1 IRC2 CLM-8 IRp60 IGSF12 CMRF35H CMRF-35H CMRF35-H CMRF35H9 CMRF35-H9 IRC1/IRC2 CMRF-35-H9 |
| Gene Full Name | CD300a molecule |
| Gene Summary | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Alternative Names | CD300A CD300a molecule CLM 8 CMRF 35 H9 CMRF 35H CMRF35 H CMRF35 H9 CMRF35 like molecule 8 CMRF35H CMRF35H9 IGSF12 Inhibitory receptor protein 60 IRC1 IRC1/IRC2 IRC2 IRp60 NK inhibitory receptor |
| Molecular Weight(MW) | 33 kDa(Observed MW 60 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD300A using anti-CD300A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of U2OS cells using anti-CD300A antibody. Overlay histogram showing U2OS cells stained with anti-CD300A antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD300A Antibody at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
