WB
Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), JK whole cell lysate(30µg), K562 whole cell lysate(30µg), Raji whole cell lysate(30µg), Mouse Spleen tissue lysate(30µg),Rat Spleen tissue lysate(30µg) All lanes: CD300A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution, Exposure time: 120s.IHC
IHC image of CD300A Antibody diluted at 1:50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.ICC/IF
Immunofluorescence staining of HepG2 cell with CD300A Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-CD300A Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human CMRF35-like molecule 8 protein (18-180AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:3000 IHC:1:50-1:200 ICC/IF:1:10-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD300A |
|---|---|
| Gene Synonyms | IRC1 IRC2 CLM-8 IRp60 IGSF12 CMRF35H CMRF-35H CMRF35-H CMRF35H9 CMRF35-H9 IRC1/IRC2 CMRF-35-H9 |
| Gene Full Name | CD300a molecule |
| Gene Summary | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Alternative Names | CD300 antigen-like family member A antibody CD300a antibody CD300a antigen antibody CD300a molecule antibody CLM-8 antibody CLM8_HUMAN antibody CMRF 35 H9 antibody CMRF-35-H9 antibody CMRF35-H antibody CMRF35-H9 antibody CMRF35-like molecule 8 antibody CMRF35H antibody CMRF35H leukocyte immunoglobulin like receptor antibody CMRF35H9 antibody IgSF12 antibody Immunoglobulin superfamily member 12 antibody Inhibitory receptor protein 60 antibody IRC1 antibody IRC1/IRC2 antibody IRC2 antibody IRp60 antibody Leukocyte membrane antigen antibody Mast cell derived paired immunoglobulin like receptor 1 antibody NK inhibitory receptor antibody Polymeric immunoglobulin receptor 4 antibody |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. |
WB
Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), HL-60 whole cell lysate(30µg), JK whole cell lysate(30µg), K562 whole cell lysate(30µg), Raji whole cell lysate(30µg), Mouse Spleen tissue lysate(30µg),Rat Spleen tissue lysate(30µg) All lanes: CD300A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution, Exposure time: 120s.
IHC
IHC image of CD300A Antibody diluted at 1:50 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
ICC/IF
Immunofluorescence staining of HepG2 cell with CD300A Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:3000 IHC:1:50-1:200 ICC/IF:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
