WB
Western blot analysis of extracts of K-562 cells, using CD300A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 90s.| Product Name | CD300A polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human CD300A(NP_009192.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:200 - 1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD300A |
|---|---|
| Gene Synonyms | IRC1 IRC2 CLM-8 IRp60 IGSF12 CMRF35H CMRF-35H CMRF35-H CMRF35H9 CMRF35-H9 IRC1/IRC2 CMRF-35-H9 |
| Gene Full Name | CD300a molecule |
| Gene Summary | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Alternative Names | CD300A CLM-8 CMRF-35-H9 CMRF-35H CMRF35-H CMRF35-H9 CMRF35H CMRF35H9 IGSF12 IRC1 IRC1/IRC2 IRC2 IRp60 |
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of extracts of K-562 cells, using CD300A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit . Exposure time: 90s.| Application Notes | WB:1:200 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
