ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using CD300A antibody against Hela(1),Cos-7(2),K562(3),THP-1(4),A549(5),Nih3t3(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Product Name | Anti-CD300A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD300A |
|---|---|
| Gene Synonyms | B230315M08Rik CLM-8 Clm8 CMRF-35-H9 CMRF-35H CMRF35-H CMRF35-H9 CMRF35H CMRF35H9 IGSF12 IRC1 IRC1/IRC2 IRC2 IRp60 LMIR1 MAIR-1 MAIR-I MAIR-Ia mcpir1 MMAC8 Pigr4 |
| Gene Full Name | CD300a molecule |
| Gene Summary | This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012] |
| Alternative Names | CD300 antigen-like family member A CD300A antigen clm-8 CMRF35H leukocyte immunoglobulin-like receptor CMRF35HIRp60 immunoglobulin superfamily member 12 inhibitory receptor protein 60 IRC1a IRC1b IRC1c leukocyte membrane antigen leukocyte mono-Ig-like receptor 1 mast cell-derived paired immunoglobulin-like receptor 1 Mcipr1 myeloid-associated immunoglobulin-like receptor 1 NK inhibitory receptor NKRL polymeric immunoglobulin receptor 4 |
| Molecular Weight(MW) | 33kDa（observed：40-60kDa) |
| Source | Rabbit |
| Cellular Localization | Cell membrane |
| SwissProt ID | Q9UGN4 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using CD300A antibody against Hela(1),Cos-7(2),K562(3),THP-1(4),A549(5),Nih3t3(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 30S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
