WB
Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody at 1/2,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane), Lane 2: 293T cell lysate (negative) (30 µg/Lane), Lane 3: HUT 102 cell lysate (30 µg/Lane), Lane 4: HeLa cell lysate (negative) (30 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IP
SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with Rabbit anti-SLAM / CD150 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-SLAM / CD150 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input), Lane 2: Rabbit anti-SLAM / CD150 antibody IP in Raji cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-SLAM / CD150 antibody in Raji cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 30 seconds.| Product Name | SLAM / CD150 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human SLAMF1 aa 1-237. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| WB:1:2000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SLAMF1 |
|---|---|
| Gene Synonyms | IPO3 SLAM CD150 CDw150 |
| Gene Full Name | signaling lymphocytic activation molecule family member 1 |
| Gene Summary | Enables SH2 domain binding activity; identical protein binding activity; and virus receptor activity. Involved in several processes, including negative regulation of CD40 signaling pathway; negative regulation of cytokine production; and positive regulation of MAPK cascade. Located in extracellular exosome. Is active in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | 4933415F16 antibody AA177906 antibody CD 150 antibody CD150 antibody CD150 antigen antibody CDw150 antibody Estm51 antibody Ipo 3 antibody IPO-3 antibody Ipo3 antibody 4933415F16 antibody AA177906 antibody CD 150 antibody CD150 antibody CD150 antigen antibody CDw150 antibody Estm51 antibody Ipo 3 antibody IPO-3 antibody Ipo3 antibody MGC151472 antibody MGC151476 antibody OTTHUMP00000025670 antibody OTTHUMP00000060252 antibody RGD1560634 antibody Signaling lymphocytic activation molecule antibody Signaling lymphocytic activation molecule family member 1 antibody Signaling lymphocytic activation molecule family member 1 isoform CRA_a antibody Signaling lymphocytic activation molecule family member 1 isoform CRA_b antibody Signaling lymphocytic activation molecule precursor antibody Slaf1 antibody SLAF1_HUMAN antibody SLAM family member 1 antibody Slamf 1 antibody SLAMF1 antibody SLAMF1 protein antibody |
| Molecular Weight(MW) | 37kDa(Observed band size: 75kDa) |
| Cellular Localization | Cell membrane; Secreted. |
WB
Western blot analysis of SLAM / CD150 on different lysates with Rabbit anti-SLAM / CD150 antibody at 1/2,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane), Lane 2: 293T cell lysate (negative) (30 µg/Lane), Lane 3: HUT 102 cell lysate (30 µg/Lane), Lane 4: HeLa cell lysate (negative) (30 µg/Lane), Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IP
SLAM / CD150 was immunoprecipitated from 0.2 mg Raji cell lysate with Rabbit anti-SLAM / CD150 antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Rabbit anti-SLAM / CD150 antibody at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Raji cell lysate (input), Lane 2: Rabbit anti-SLAM / CD150 antibody IP in Raji cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-SLAM / CD150 antibody in Raji cell lysate, Blocking/Dilution buffer: 5% NFDM/TBST, Exposure time: 30 seconds.| Application Notes | WB:1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
