WB
Western blot analysis of MSR1 / CD204 on different lysates with Rabbit anti-MSR1 / CD204 antibody at 1/5,000 dilution.
Lane 1: J774A.1 (Mouse monocyte macrophages) cell lysate,
Lane 2: Neuro-2a (Mouse brain neuroblastoma cells) cell lysate,
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute;
Neuro-2a is a negative control.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
WB
Western blot analysis of MSR1 / CD204 on mouse liver tissue lysate with Rabbit anti-MSR1 / CD204 antibody at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes;
Blocking: 5% NFDM/TBST, 1 hour at room temperature,
Primary antibody:1/5,000 in primary antibody dilution buffer, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature