WB
Western blot analysis of LXRα/β expression in THP-1 (A), c4 (B), M1 (C),A549 (D) and U-87 MG (E) whole cell lysates.WB
Western blot analysis of LXRα/β expression in whole-cell lysates of HeLa (A) and THP-1 (B) cells.ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Product Name | Mouse Monoclonal antibody to LXR alpha/beta/NR1H3/NR1H2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | amino acids 433-461 at the C-terminus of LXRb of human origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1H2 |
|---|---|
| Gene Synonyms | NER UNR LXRB LXR-b NER-I RIP15 |
| Gene Full Name | nuclear receptor subfamily 1 group H member 2 |
| Gene Summary | The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010] |
| Molecular Weight(MW) | 50 kDa,56 kDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of LXRα/β expression in THP-1 (A), c4 (B), M1 (C),A549 (D) and U-87 MG (E) whole cell lysates.
WB
Western blot analysis of LXRα/β expression in whole-cell lysates of HeLa (A) and THP-1 (B) cells.
ICC/IF
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.| Application Notes | WB:1:100-1:1000 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
