WB
Primary: Anti-phospho-Parkin (Ser65) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionIHC
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | phospho-Parkin (Ser65) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human Parkin around the phosphorylation site of Ser65 : QQ(p-S)IV |
| Modification | p-Ser65 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | PDJ AR-JP LPRS2 PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Alternative Names | AR-JP LPRS2 PARK2 PDJ PRKN_HUMAN PRKN Parkin Parkin RBR E3 ubiquitin-protein ligase Parkinson juvenile disease protein 2 (Parkinson disease protein 2) 2.3.2.31 parkin RBR E3 ubiquitin protein ligase Parkinson disease (autosomal recessive juvenile) 2 parkin parkinson protein 2 E3 ubiquitin protein ligase (parkin) E3 ubiquitin ligase PRKN | Parkin (phospho-S65) p-Parkin phospho-Parkin p-PRKN PRKN | Parkin (phospho-Ser65) |
| Molecular Weight(MW) | 52 kDa(Observed band size: 50 kDa) |
| Cellular Localization | Cell projection Cytoplasm,Endoplasmic reticulum,Membrane,Mitochondrion,Mitochondrion outer membrane ,Nucleus,Synapse |
WB
Primary: Anti-phospho-Parkin (Ser65) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
IHC
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Parkin (Ser65)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
