WB
Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500IHC
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Parkin (Phospho-Ser131) Antibody. The picture on the right is blocked with the phospho peptide.ELISA
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Parkin (Phospho-Ser131) Antibody| Product Name | Parkin (Phospho Ser131) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Parkin around the phosphorylation site of Ser131. AA range:101-150 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 IF:1:50-1:200 ELISA:1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | PDJ AR-JP LPRS2 PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Alternative Names | PARK2 PRKN E3 ubiquitin-protein ligase parkin Parkinson juvenile disease protein 2 Parkinson disease protein 2 |
| Molecular Weight(MW) | 51kD |
| Cellular Localization | Cell projection,Cytoplasm,Endoplasmic reticulum,Membrane,Mitochondrion |
WB
Western blot analysis of 293T AD293 Hela using p-Parkin (S131) antibody. Antibody was diluted at 1:500
IHC
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Parkin (Phospho-Ser131) Antibody. The picture on the right is blocked with the phospho peptide.
ELISA
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Parkin (Phospho-Ser131) Antibody| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 IF:1:50-1:200 ELISA:1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
