WB
Parkin: Western blot analysis of Parkin expression in SH-SY5Y whole cell lysate (A) and mouse brain tissue extract (B).WB
Parkin Antibody: Near‑infrared Western blot analysis of Parkin expression in rat brain tissue extract. Blots were blocked with UltraCruz® Blocking Reagent. Detection reagent used: m‑IgGκ BP‑CFL 680.IHC
Parkin: Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and nuclear staining of glandular cells at low (A) and high (B) magnification.Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | Mouse Monoclonal antibody to Parkin |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against recombinant human Parkin with an epitope mapping between amino acids 399-465 at the C-terminus within the RING2/R2, second RIND domain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKN |
|---|---|
| Gene Synonyms | PDJ AR-JP LPRS2 PARK2 |
| Gene Full Name | parkin RBR E3 ubiquitin protein ligase |
| Gene Summary | The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 50-58 kDa |
| Cellular Localization | Cell projection,Cytoplasm,Endoplasmic reticulum,Membrane,Mitochondrion ,Mitochondrion outer membrane,Nucleus,Synapse |
WB
Parkin: Western blot analysis of Parkin expression in SH-SY5Y whole cell lysate (A) and mouse brain tissue extract (B).
WB
Parkin Antibody: Near‑infrared Western blot analysis of Parkin expression in rat brain tissue extract. Blots were blocked with UltraCruz® Blocking Reagent. Detection reagent used: m‑IgGκ BP‑CFL 680.
IHC
Parkin: Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and nuclear staining of glandular cells at low (A) and high (B) magnification.Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
